A Novel Integrative Approach to Oral Microbiome Dysbiosis in a Patient with Polycystic Ovary Syndrome Using Pulsatilla 1M: A Pilot Case Report

Jayasree V; Sonnymon R; Rachana Mol R S; Smera Eldhose; Ramya J K

A Novel Integrative Approach to Oral Microbiome Dysbiosis in a Patient with Polycystic Ovary Syndrome Using Pulsatilla 1M: A Pilot Case Report

Author(s)	Dr. Jayasree V, Sonnymon R, Rachana Mol R S, Smera Eldhose, Ramya J K
Country	India
Abstract	Background: Polycystic Ovary Syndrome (PCOS) is a prevalent endocrine disorder affecting women of reproductive age and is associated with systemic complications such as insulin resistance, obesity, and cardiovascular disease. Emerging evidence suggests that PCOS may also influence the composition of the oral microbiome, potentially compounding oral health problems, yet this relationship remains largely unexplored. A prior systematic review of the impact of PCOS on the oral microbiome demonstrated a significant association between the two, underscoring the role of dysbiosis in both oral and systemic health. This study aims to evaluate the effect of the homoeopathic remedy Pulsatilla 1M on the oral microbiome of a woman diagnosed with PCOS. Methodology: Oral swabs were collected from the patient before and after administration of Pulsatilla 1M. Samples were serially diluted, plated on nutrient agar, and incubated at 37°C for 24–48 hours. Colony Forming Units (CFUs) were enumerated, isolates were purified by the streak-plate method, and microbial identification was performed using Gram staining and standard biochemical tests (indole, methyl red, Voges–Proskauer, and citrate utilisation). Results: Pre-intervention cultures showed heavy, confluent growth (10⁻² dilution: too numerous to count; 10⁻³ dilution: 189 CFU) with a mixed flora of Streptococcus species (Gram-positive cocci) and Pseudomonas species (Gram-negative bacilli; citrate-positive). Post-intervention, colony counts fell markedly (10⁻² dilution: 143 CFU; 10⁻³ dilution: 18 CFU) — an approximate 3-log reduction in bacterial load — with only occasional Streptococcus colonies detected and Pseudomonas species no longer isolated. Discussion: The reduction in pathogenic colony counts and the disappearance of Pseudomonas species following Pulsatilla 1M administration suggest a possible modulatory effect on oral microbial homeostasis, plausibly mediated through systemic hormonal and immune regulation. These preliminary findings offer a new perspective on the potential role of homoeopathy in addressing microbiome alterations linked to endocrine disorders such as PCOS. Conclusion: This pilot case study provides preliminary evidence that Pulsatilla 1M may help restore oral microbial balance in PCOS, supporting the case for larger, controlled, integrative studies.
Keywords	Polycystic Ovary Syndrome; Oral Microbiome; Pulsatilla; Dysbiosis; Homoeopathy; Pilot Study
Discipline	Other
Published In	Volume 4, Issue 5, September-October 2026
Published On	2026-10-06

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A Novel Integrative Approach to Oral Microbiome Dysbiosis in a Patient with Polycystic Ovary Syndrome Using Pulsatilla 1M: A Pilot Case Report

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